您的浏览器目前处于缩放状态,页面可能会出现错位现象,建议100%大小显示。(快捷键:Ctrl+0)

当前位置:首页 > 图像设计 > 发表快报

Small Methods - 中国地质大学(武汉)夏帆教授、娄筱叮教授

发布时间: 2020-2-15 来源: WILEY-VCH

Title: Enzyme and AIEgens Modulated Solid‐State Nanochannels: In Situ and Noninvasive Monitoring of H2O2 Released from Living Cells

Abstract: Solid‐state nanochannels have revealed great abilities in the sensing of ions, small biomolecules, and biological macromolecules. However, the current platform requires pretreatment of real samples to extract targets, which may induce false signals due to complicated collection and addition processes. Although nanopore electrodes or nanopipettes have been successfully utilized in the detection of intracellular redox‐active species without sample preparation processes, the insertion of nanoprobes to living cells is inevitable. Here, a strategy is reported to monitor H2O2 released from living cells based on functionalized solid‐state nanochannels without an insertion procedure. In this strategy, aggregation‐induced emission luminogens (AIEgens) with enzyme‐responsive linkage properties are combined in solid‐state nanochannels. When H2O2 released from cervical cancer cells (HeLa), Tyr‐containing AIEgens (TT) will form horseradish peroxidase‐modulated (HRP‐modulated) linkages in the nanochannels. The formation of linkages can result in the effective blockade of nanochannels, hence via transmembrane ionic current. Owing to the aggregation of linkage products, a fluorescence signal can be observed. By using these dual‐signal‐output nanochannels, in situ and noninvasive detection of H2O2 is able to be achieved. Together with molecular dynamic (MD) simulations results, solid surface zeta potential and contact angle experiments, it is concluded that the blockage of nanochannels is the dominate factor for ionic currents as well as fluorescence change.

客服:400-070-3060